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KMID : 0385520080210050424
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Analytical Science & Technology
2008 Volume.21 No. 5 p.424 ~ p.431
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Analysis of streptomycin in honey by LC-MS/MS
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Shim Young-Eun
Myung Seung-Woon
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Abstract
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Streptomycin, which is one of aminoglycoside antibiotics, has been widely used in the rearing of foodproducing animals to prevent and treat diseases in cattle, pigs and poultry. Although not licensed in South Korea, streptomycin has also been used for the treatment of bacterial honeybee disease, such as European foulbrood in Third World countries. A reliable and effective method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of streptomycin in honey. A established method was optimized the clean-up and extraction procedure for the trace determination, good precision and accuracy. And the chromatographic and tandem mass spectrometric parameters were also optimized. The precision (RSD) and accuracy (bias) in the concentration range of 5.0~50.0 ug/kg were 5.5~14% and -10.0~8.0%, respectively. Limit of detection was 0.75 ug/kg and recovery of streptomycin spiked at level of 10 ug/kg in honey was 74%. The established and validated method was applied to determine streptomycin in honey which was on the market.
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KEYWORD
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Streptomycin, antibiotics, LC-MS/MS, honey
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